Application
Western blot analysis of BMF using| Product Name | BMF antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to BMFBmf (for Bcl-2-modifying factor) is a novel BH3-only protein recently identified in human and mouse. The BH3 domain in Bmf is required for both binding to Bcl-2 proteins and for triggering apoptosis. In healthy cells, Bmf associates with the dynein light chain 2 (DLC2) component of the myosin V motors and is sequestered by the cell's actin cytoskeleton. Disruption of the actin cytoskeleton, either by depolymerization of actin filaments or by detachment of cells from the extracellular matrix, triggers release and activation of Bmf, initiating the downstream apoptosis program. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| Western blotting (0.5-4 µg/ml). However, the optimal conditions should be determined individually. The antibody recognizes ~28 kDa BMF in sample from human, mouse and rat origins. Reactivity to other species has not been tested. | |
| Species Reactivity | HumanMouseRat |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | Bcl-2-modifying factor, FLJ00065, Bcl2modifying factor, BMF, Bcl 2 modifying factor |
|---|---|
| Target | BMF |
| Tissue Specificity | This antibody is specific for BMF. |
Application
Western blot analysis of BMF using| Application Notes | Western blotting (0.5-4 µg/ml). However, the optimal conditions should be determined individually. The antibody recognizes ~28 kDa BMF in sample from human, mouse and rat origins. Reactivity to other species has not been tested. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | The antibody solution should be gently mixed before use. Stored at -20 °C for future use. |
| Storage Buffer | PBS (pH 7.2) containing 30% glycerol, 0.5% BSA, and 0.01% thimerosal |
