Application
Bmi1 antibody detects Bmi1 protein by western blot analysis. A. 30 μg K562 whole cell lysate/extract B. 30 μg THP-1 whole cell lysate/extract 10 % SDS-PAGE Bmi1 antibody dilution: 1:3000Application
Bmi1 antibody immunoprecipitates Bmi1 protein in IP experiments. IP samples: K562 whole cell extract A. 40 μg K562 whole cell extract B. Control with 4 μg of preimmune Rabbit IgG C. Immunoprecipitation of Bmi1 protein by 4 μg Bmi1 antibody 5 % SDS-PAGE The immunoprecipitated Bmi1 protein was detected by Bmi1 antibody diluted at 1:500. [EasyBlot anti-rabbit IgG was used as a secondary reagent]| Product Name | Bmi1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Bmi1 (BMI1 polycomb ring finger oncogene)Component of the Polycomb group (PcG) multiprotein PRC1 complex, a complex required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility. In the PRC1 complex, it is required to stimulate the E3 ubiquitin-protein ligase activity of RNF2/RING2. |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human Bmi1. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IPWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Xenopus laevis, Dog, Cat, Pig, Chicken, Chimpanzee, BovineMouse (96%), Rat (92%), Xenopus laevis (82%), Dog (98%), Cat (99%), Pig (99%), Chicken (94%), Chimpanzee (100%), Bovine (98%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | FLVI2/BMI1, PCGF4, BMI1, BMI1 polycomb ring finger oncogene, RNF51 |
|---|---|
| Target | Bmi1 |
| Molecular Weight(MW) | 37 kDa (note) |
| Cellular Localization | Nucleus , Cytoplasm |
Application
Bmi1 antibody detects Bmi1 protein by western blot analysis. A. 30 μg K562 whole cell lysate/extract B. 30 μg THP-1 whole cell lysate/extract 10 % SDS-PAGE Bmi1 antibody dilution: 1:3000
Application
Bmi1 antibody immunoprecipitates Bmi1 protein in IP experiments. IP samples: K562 whole cell extract A. 40 μg K562 whole cell extract B. Control with 4 μg of preimmune Rabbit IgG C. Immunoprecipitation of Bmi1 protein by 4 μg Bmi1 antibody 5 % SDS-PAGE The immunoprecipitated Bmi1 protein was detected by Bmi1 antibody diluted at 1:500. [EasyBlot anti-rabbit IgG was used as a secondary reagent]| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
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| Form | Liquid |
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| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
