Application
Sample (30 µg of whole cell lysate) A: NIH-3T3 B: JC 10% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Immunohistochemical analysis of paraffin-embedded MDA-MB468 xenograft, using BMP4 antibody at 1:100 dilution.Application
BMP4 antibody detects BMP4 protein by western blot analysis. A. 30 µg A549 whole cell lysate/extract 10% SDS-PAGE BMP4 antibody dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Immunofluorescence analysis of methanol-fixed HeLa, using BMP4 antibody at 1:100 dilution.| Product Name | BMP4 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to BMP4 (bone morphogenetic protein 4)The protein encoded by this gene is a member of the bone morphogenetic protein family which is part of the transforming growth factor-beta superfamily. The superfamily includes large families of growth and differentiation factors. Bone morphogenetic proteins were originally identified by an ability of demineralized bone extract to induce endochondral osteogenesis in vivo in an extraskeletal site. This particular family member plays an important role in the onset of endochondral bone formation in humans, and a reduction in expression has been associated with a variety of bone diseases, including the heritable disorder Fibrodysplasia Ossificans Progressiva. Alternative splicing in the 5' untranslated region of this gene has been described and three variants are described, all encoding an identical protein. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human BMP4. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rat, Dog, Pig, Rabbit, Sheep, BovineRat (97%), Dog (98%), Pig (98%), Rabbit (96%), Sheep (98%), Bovine (98%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | BMP2B antibody, BMP2B1 antibody, MCOPS6 antibody, OFC11 antibody, ZYME antibody, BMP4 antibody, BMP-2B antibody, bone morphogenetic protein 4 antibody, bone morphogenetic protein 2B antibody, BMP-4 antibody |
|---|---|
| Molecular Weight(MW) | 47 kDa (note) (3) |
| Cellular Localization | Secreted , extracellular space , extracellular matrix |
Application
Sample (30 µg of whole cell lysate) A: NIH-3T3 B: JC 10% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Immunohistochemical analysis of paraffin-embedded MDA-MB468 xenograft, using BMP4 antibody at 1:100 dilution.
Application
BMP4 antibody detects BMP4 protein by western blot analysis. A. 30 µg A549 whole cell lysate/extract 10% SDS-PAGE BMP4 antibody dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Immunofluorescence analysis of methanol-fixed HeLa, using BMP4 antibody at 1:100 dilution.| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
