| Product Name | BMP4 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [66119.11] to BMP 4Bone Morphogenic Proteins (BMP) are members of the TGF-beta superfamily that affect bone and cartilage formation (1,2,3). Mature BMPs are 30-38 kDa proteins that assume a TGF-beta -like cysteine knot configuration. Lovostatin increases bone formation by turning on the bmp-2 gene (4). BMPs stimulate the production of specific bone matrix proteins and alter stromal cell and osteoclast proliferation (5,6). BMPs may also be an important factor for development of the viscera, with roles in cell proliferation, apoptosis, differentiation, and morphogenesis (1,7). BMPs appear to be responsible for normal dorsal/ventral patterning. Like TGF-beta, BMPs bind to a type II receptor, which then recruits the transducing type I receptor unit, activating the Smad protein signaling pathway (8,9,10). |
| Immunogen | Recombinant full length protein expressed in NSO cells (Human). |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2 |
| Clone Number | 66119.11 |
| Host Species | Mouse |
| Tested Applications | ELISANeutWB |
| I-ELISA: Use at a concentration of 0.5 - 1.0 μg/ml detects a limit of around 6 ng/well of human BMP-4. Neut: Use at a concentration of 1 - 3 μg/ml to neutralise 50% of the bioactivity due to 0.5 μg/ml recombinant human BMP-4I-WB: Use at a concentration of 1 - 2 μg/ml (using human BMP-4 at 50 ng/lane under non-reducing and reducing conditions). Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | Human |
| Concentration | 0.5mg/ml |
| Purification | Protein G |
| Gene Synonyms | BMP4 BMP2B1 BMP2B P12644 652 112262 ZYME |
|---|---|
| Target | BMP4 |
| Tissue Specificity | Monoclonal Anti-BMP-4 neutralizes human BMP-4, and recognizes recombinant human BMP-4 by ELISA and immunoblotting. The antibody shows approximately 5% cross-reactivity with recombinant human BMP-2, recombinant mouse BMP RIA and BMP-RIB. |
| Application Notes | I-ELISA: Use at a concentration of 0.5 - 1.0 μg/ml detects a limit of around 6 ng/well of human BMP-4. Neut: Use at a concentration of 1 - 3 μg/ml to neutralise 50% of the bioactivity due to 0.5 μg/ml recombinant human BMP-4I-WB: Use at a concentration of 1 - 2 μg/ml (using human BMP-4 at 50 ng/lane under non-reducing and reducing conditions). Optimal dilutions/concentrations should be determined by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
