Application
Immunohistochemical analysis of paraffin-embedded U87 xenograft, using BNIP2 antibody at 1:500 dilution.Application
BNIP2 antibody detects BNIP2 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with BNIP2 antibody diluted by 1:1000.Application
Sample (30 ug of whole cell lysate) A: HepG2 10% SDS PAGE diluted at 1:1000| Product Name | BNIP2 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to BNIP2 (BCL2/adenovirus E1B 19kDa interacting protein 2)This gene is a member of the BCL2/adenovirus E1B 19 kd-interacting protein (BNIP) family. Though the specific function is unknown, it interacts with the E1B 19 kDa protein which is responsible for the protection of virally-induced cell death, as well as E1B 19 kDa-like sequences of BCL2, also an apoptotic protector. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human BNIP2. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Zebrafish, Xenopus laevis, BovineMouse (91%), Rat (92%), Zebrafish (81%), Xenopus laevis (86%), Bovine (97%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | BNIP-2 antibody NIP2 antibody BNIP2 antibody BCL2/adenovirus E1B 19 kDa protein-interacting protein 2 antibody BCL2/adenovirus E1B 19kDa interacting protein 2 antibody |
|---|---|
| Molecular Weight(MW) | 36 kDa (note) (4) |
| Cellular Localization | Cytoplasm , perinuclear region |
Application
Immunohistochemical analysis of paraffin-embedded U87 xenograft, using BNIP2 antibody at 1:500 dilution.
Application
BNIP2 antibody detects BNIP2 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with BNIP2 antibody diluted by 1:1000.
Application
Sample (30 ug of whole cell lysate) A: HepG2 10% SDS PAGE diluted at 1:1000| Positive Control | A431 , HeLa , HepG2 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
