Application
Sample (50 ug of whole cell lysate) A: mouse liver 12% SDS PAGE diluted at 1:500Application
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using BNIP3L antibody at 1:500 dilution.Application
Sample (30 ug of whole cell lysate) A: Molt-4 12% SDS PAGE diluted at 1:1000Application
Immunofluorescence analysis of methanol-fixed HeLa, using BNIP3L antibody at 1:200 dilution.| Product Name | BNIP3L antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to BNIP3L (BCL2/adenovirus E1B 19kDa interacting protein 3-like)This gene is a member of the BCL2/adenovirus E1B 19 kd-interacting protein (BNIP) family. It interacts with the E1B 19 kDa protein which is responsible for the protection of virally-induced cell death, as well as E1B 19 kDa-like sequences of BCL2, also an apoptotic protector. The protein encoded by this gene is a functional homolog of BNIP3, a proapoptotic protein. This protein may function simultaneously with BNIP3 and may play a role in tumor suppression. [provided by RefSeq] |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of human BNIP3L. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanMouseBovine |
| Predicted Reactivity | Rat, Chicken, Rhesus MonkeyRat (100%), Chicken (100%), Rhesus Monkey (100%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | BNIP3a antibody NIX antibody BNIP3L antibody BCL2/adenovirus E1B 19-kd protein-interacting protein 3a antibody NIP3-like protein X antibody BCL2/adenovirus E1B 19 kDa protein-interacting protein 3A antibody adenovirus E1B19k-binding protein B5 antibo |
|---|---|
| Molecular Weight(MW) | 24 kDa (note) (2) |
| Cellular Localization | Nucleus envelope , Endoplasmic reticulum , Mitochondrion , Membrane |
Application
Sample (50 ug of whole cell lysate) A: mouse liver 12% SDS PAGE diluted at 1:500
Application
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using BNIP3L antibody at 1:500 dilution.
Application
Sample (30 ug of whole cell lysate) A: Molt-4 12% SDS PAGE diluted at 1:1000
Application
Immunofluorescence analysis of methanol-fixed HeLa, using BNIP3L antibody at 1:200 dilution.| Positive Control | HeLa , Molt-4 , Raji , mouse liver |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
