Application
BNP antibody detects BNP protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with BNP antibody diluted by 1:1000.Application
BNP antibody [N1C3] detects BNP protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: BNP protein stained by BNP antibody [N1C3] diluted at 1:500. Blue: Hoechst 33342 staining.| Product Name | BNP antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to BNP (natriuretic peptide precursor B)This gene is a member of the natriuretic peptide family and encodes a secreted protein which functions as a cardiac hormone. The protein undergoes two cleavage events, one within the cell and a second after secretion into the blood. The protein's biological actions include natriuresis, diuresis, vasorelaxation, inhibition of renin and aldosterone secretion, and a key role in cardiovascular homeostasis. A high concentration of this protein in the bloodstream is indicative of heart failure. Mutations in this gene have been associated with postmenopausal osteoporosis. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human BNP. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | BNP antibody NPPB antibody natriuretic peptides B antibody natriuretic peptide precursor B antibody gamma-brain natriuretic peptide antibody brain type natriuretic peptide antibody natriuretic protein antibody natriuretic peptide B antibody |
|---|---|
| Target | BNP |
| Molecular Weight(MW) | 15 kDa (note) |
| Cellular Localization | Secreted |
Application
BNP antibody detects BNP protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with BNP antibody diluted by 1:1000.
Application
BNP antibody [N1C3] detects BNP protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: BNP protein stained by BNP antibody [N1C3] diluted at 1:500. Blue: Hoechst 33342 staining.| Positive Control | 293T , A431 , HeLa , HepG2 |
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| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
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| Form | Liquid |
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| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
