| Product Name | BNP antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to BNPBrain natriuretic peptide (BNP) circulates in blood as a peptide hormone with natriuretic, vasodilatory and renin inhibitory properties. BNP is secreted predominantly by the left ventricular myocytes in response to volume expansion and pressure overload. BNP belongs to a family of structurally similar peptide hormones, which includes atrial natriuretic peptide (ANP), BNP, C-type natriuretic peptide (CNP) and urodilatin. These peptides are characterized by a common 17 amino acid ring structure with a disulfide bond between two cystein residues. This ring structure shows high homology between different natriuretic peptides (eleven of the 17 amino acid residues are homologous in the ring of each of the natriuretic peptides, see fig. 18). BNP is a 32 amino acid peptide with disulfide bond between the cystein residues Cys10 and Cys26. In earlier studies it has been demonstrated that BNP concentration in blood increases with the severity of congestive heart failure. Quantitative measurement of BNP in blood provides an objective indicator of congestive heart failure severity |
| Immunogen | Synthetic human Brain Natriuretic Polypeptide covalently attached to a carrier protein (bovine thyroglobulin). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISA,IHC,RIA |
| ELISA: Use at an assay dependent dilution. IHC: 1:40. Has been found to stain 3% buffered paraformaldehyde fixed rat brain sections using indirect immunofluorescent procedure at a dilution of 1:1000. RIA: Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the end user. | |
| Species Reactivity | Human,Rat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | P16860, 600295, BNP, NPPB, 4879 |
|---|---|
| Target | BNP |
| Cellular Localization | Secreted |
| Light Chain Type | n/a |
| Application Notes | ELISA: Use at an assay dependent dilution. IHC: 1:40. Has been found to stain 3% buffered paraformaldehyde fixed rat brain sections using indirect immunofluorescent procedure at a dilution of 1:1000. RIA: Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the end user. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4°C short term. For extended storage aliquot and store at -20°C or below. Avoid freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
