Application
Western blot analysis of POU2AF1 Antibody (N-term) in Ramos cell line lysates (35ug/lane). POU2AF1 (arrow) was detected using the purified Pab.Application
POU2AF1 Antibody (N-term) flow cytometry analysis of Ramos cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
POU2AF1 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human tonsil tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of POU2AF1 Antibody (N-term) for immunohistochemistry.| Product Name | BOB1 antibody, N-term |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to BOB1 (N-term)Transcriptional coactivator that specifically associates with either OCT1 or OCT2. It boosts the OCT1 mediated promoter activity and to a lesser extent, that of OCT2. It has no intrinsic DNA-binding activity. It recognizes the POU domains of OCT1 and OCT2. It is essential for the response of B-cells to antigens and required for the formation of germinal centers. |
| Immunogen | KLH conjugated synthetic peptide between 7~36 amino acids from the N-terminal region of human BOB1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| ELISA: Use at 1:1,000: |
|
| Species Reactivity | Human |
| Concentration | 0.2mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | POU domain class 2-associating factor 1 BOB-1 OBF1 POU2AF1 POU2AF1 OCT-binding factor 1 B-cell-specific coactivator OBF-1 OCAB OCTbinding factor1 Bcellspecific coactivator OBF1 OCTbinding factor 1 BOB1 POU domain class2associating factor1 POU |
|---|---|
| Target | BOB1 |
| Molecular Weight(MW) | 27.436 kDa (note) |
Application
Western blot analysis of POU2AF1 Antibody (N-term) in Ramos cell line lysates (35ug/lane). POU2AF1 (arrow) was detected using the purified Pab.
Application
POU2AF1 Antibody (N-term) flow cytometry analysis of Ramos cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
POU2AF1 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human tonsil tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of POU2AF1 Antibody (N-term) for immunohistochemistry.| Application Notes | ELISA: Use at 1:1,000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at at 2-8°C for up to 6 months. For long term storage, aliquot and store at -20 °C. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.09% (W/V) sodium azide |
