Application
Immunofluorescence of Bora in Mouse Brain cells with Bora antibody at 20 ug/mL.Application
Western blot analysis of Bora in Jurkat cell lysate with Bora antibody at (A) 1 and (B) 2 ug/ml.Application
Immunohistochemistry of Bora in mouse brain tissue with Bora antibody at 2.5 ug/ml.| Product Name | Bora antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to BoraBora (Protein aurora borealis) is a key activator of Aurora Related Protein Kinase A (ARK-1), which is a centrosome-associated serine/threonine kinase that regulates centrosome maturation, bipolar spindle assembly and chromosome segregation during mitosis. Bora is localized to the nucleus until mitosis is initiated, then translocates to the cytoplasm in a Cdc2 dependent manner. Activation of Cdc2 initiates the release of Bora into the cytoplasm where it can bind and activate ARK-1. PLK1 (polo-like kinase-1) interacts with Bora to control the accessibility of its activation loop for phosphorylation and activation by ARK-1. Bora and ARK-1 cooperatively activate PLK1 and control mitotic entry. Bora mutants result in multipolar spindles in mitosis identical to those observed when ARK-1 function is blocked. Thus, the ARK1-Bora-PLK1 regulatory circuit in mammalian cells elucidates a key mechanism in cell cycle regulation. At least three isoforms of Bora are known to exist. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAICC/IFIHC-PWB |
| ICC/IF:1:200~1:500 IHC-P:1:50~1:200 WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | Bora Chromosome13 open reading frame34 C13orf34 Chromosome 13 open reading frame 34 Protein aurora borealis |
|---|---|
| Target | Bora |
| Tissue Specificity | This antibody is specific for Bora N-Terminus |
Application
Immunofluorescence of Bora in Mouse Brain cells with Bora antibody at 20 ug/mL.
Application
Western blot analysis of Bora in Jurkat cell lysate with Bora antibody at (A) 1 and (B) 2 ug/ml.
Application
Immunohistochemistry of Bora in mouse brain tissue with Bora antibody at 2.5 ug/ml.| Positive Control | Blocking Peptide: |
|---|---|
| Application Notes | ICC/IF:1:200~1:500 IHC-P:1:50~1:200 WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | PBS containing 0.02% sodium azide |
