Application
Immunofluorescence of Bora in Mouse Brain cells with Bora antibody at 20 μg/mL.Application
Immunohistochemistry of Bora in mouse brain tissue with Bora antibody at 2.5 μg/mL.Application
Western blot analysis of Bora in mouse brain tissue lysate with Bora antibody at (A) 1 and (B) 2 μg/mL.| Product Name | Bora Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Bora Antibody: C13orf34, C13orf34, Protein aurora borealis, HsBora |
| Product description | Bora Antibody: Bora (Protein aurora borealis) is a key activator of Aurora Related Protein Kinase A (ARK-1), which is a centrosome-associated serine/threonine kinase that regulates centrosome maturation, bipolar spindle assembly and chromosome segregation during mitosis. Bora is localized to the nucleus until mitosis is initiated, then translocates to the cytoplasm in a Cdc2 dependent manner. Activation of Cdc2 initiates the release of Bora into the cytoplasm where it can bind and activate ARK-1. PLK1 (polo-like kinase-1) interacts with Bora to control the accessibility of its activation loop for phosphorylation and activation by ARK-1. Bora and ARK-1 cooperatively activate PLK1 and control mitotic entry. Bora mutants result in multipolar spindles in mitosis identical to those observed when ARK-1 function is blocked. Thus, the ARK1-Bora-PLK1 regulatory circuit in mammalian cells elucidates a key mechanism in cell cycle regulation. At least three isoforms of Bora are known to exist.1) Berdnik D and Knoblich JA. Drosophila Aurora A is required for centrosome maturation and Actin-dependent asymmetric protein localization during mitosis. Curr. Biol.2002; 12:640-647. |
| Immunogen | Bora antibody was raised against a 15 amino acid synthetic peptide from near the carboxy terminus of human Bora. The immunogen is located within amino acids 410 - 460 of Bora. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| Bora antibody can be used for detection of Bora by Western blot at 1-2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
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| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | C13orf34 |
|---|---|
| Alternative Names | Bora Antibody: C13orf34 C13orf34 Protein aurora borealis HsBora |
Application
Immunofluorescence of Bora in Mouse Brain cells with Bora antibody at 20 μg/mL.
Application
Immunohistochemistry of Bora in mouse brain tissue with Bora antibody at 2.5 μg/mL.
Application
Western blot analysis of Bora in mouse brain tissue lysate with Bora antibody at (A) 1 and (B) 2 μg/mL.| Positive Control | 1) Cat. No. 1403 - Mouse Brain Tissue Lysate |
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| Application Notes | Bora antibody can be used for detection of Bora by Western blot at 1-2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Bora antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Bora Antibody is supplied in PBS containing 0.02% sodium azide. |
