Rabbit polyclonal antibody to BORG3Probably involved in the organization of the actin cytoskeleton. May act downstream of CDC42 to induce actin filament assembly leading to cell shape changes. Induces pseudopodia formation in fibroblasts. Inhibits MAPK8 independently of CDC42 binding. Controls septin organization and this effect is negatively regulated by CDC42.
Immunogen
The antiserum was produced against synthesized peptide derived from human BORG3.
Key Feature
Clonality
Polyclonal
Host Species
Rabbit
Tested Applications
ELISAWB
ELISA: 1:40000Optimal dilutions should be determined experimentally by the end user.:
Species Reactivity
HumanMouse
Concentration
1mg/ml
Purification
Affinity purified
Target Information
Gene Synonyms
Cdc42 effector protein 5 Binder of Rho GTPases 3 Binder of Rho GTPases3 BORG3 Cdc42 effector protein5
Target
BORG3
Molecular Weight(MW)
15 kDa (note)
Tissue Specificity
BORG3 Antibody detects endogenous levels of total BORG3 protein.
Application
Application
Western blot analysis of extracts from Jurkat cells, using BORG3 Antibody. The lane on the right is treated with the synthesized peptide.
Application Notes
ELISA: 1:40000Optimal dilutions should be determined experimentally by the end user.:
Additional Information
Form
Liquid
Storage Instructions
Store at -20 degrees Celcius. Product is stable for at least 1 year.
Storage Buffer
Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.