Application
Western blot analysis of Boris expression in lysate from Jurkat cells (Lane 1 & 2), 3T3 cells (Lane 3) and Rat kidney (Lane 4).| Product Name | Boris antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to BorisBrother of the regulator of imprinted sites (BORIS) is a transcription factor also known as CCCTC- binding factor like protein (CTCF). Boris is usually expressed at a high level in the testis which correlates with male germ cell development and is also abnormally activated in a broad range of human cancers. Boris can be found in the nucles as well as in the cytoplasm. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| Western blotting (0.5-4 µg/ml). However, the optimal conditions should be determined individually. Other applications have not been tested. The antibody detects ~76 kDa of Boris in samples from human, mouse and rat origins.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | CTCF CTCF paralog Brother of the regulator of imprinted sites Zinc finger protein CTCFT Zinc finger protein CTCF-T CCCTC-binding factor Transcriptional repressor CTCFL CTCFL CCCTCbinding factor CTCF T CCCTC binding factor Zinc finger protein CTC |
|---|---|
| Target | Boris |
| Tissue Specificity | This antibody is specific for Boris. |
| Cellular Localization | May translocate to the nucleolus upon cell differentiation. Associates with both centromeres and chromosomal arms during metaphase. Associates with the H19 ICR in mitotic chromosomes. May be preferentially excluded from heterochromatin during interphase. |
Application
Western blot analysis of Boris expression in lysate from Jurkat cells (Lane 1 & 2), 3T3 cells (Lane 3) and Rat kidney (Lane 4).| Application Notes | Western blotting (0.5-4 µg/ml). However, the optimal conditions should be determined individually. Other applications have not been tested. The antibody detects ~76 kDa of Boris in samples from human, mouse and rat origins.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | The antibody solution should be gently mixed before use. Stored at -20 °C for future use. |
| Storage Buffer | PBS (pH 7.2) containing 30% glycerol and 0.01% thimerosal |
