Application
Western blot analysis of BPI Antibody (Center) in HL-60 cell line lysates (35ug/lane). BPI (arrow) was detected using the purified Pab.Application
BPI Antibody (Center) flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Formalin-fixed and paraffin-embedded human tonsil tissue reacted with BPI Antibody (Center) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.| Product Name | BPI antibody, Internal |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to BPI (Internal)This gene encodes a lipopolysaccharide binding protein. It is associated with human neutrophil granules and has bactericidal activity on gram-negative organisms. [provided by RefSeq] |
| Immunogen | KLH conjugated synthetic peptide between 301~331 amino acids from the Center region of human BPI. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| ELISA: Use at 1:1,000: |
|
| Species Reactivity | Human |
| Concentration | 0.2mg/ml |
| Gene Synonyms | BPI CAP57 Bactericidal permeability-increasing protein BPI Bactericidal permeabilityincreasing protein BPI CAP 57 |
|---|---|
| Target | BPI |
| Molecular Weight(MW) | 53.9 kDa (note) |
| Cellular Localization | Membrane-associated in polymorphonuclear Leukocytes (PMN) granules. |
Application
Western blot analysis of BPI Antibody (Center) in HL-60 cell line lysates (35ug/lane). BPI (arrow) was detected using the purified Pab.
Application
BPI Antibody (Center) flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Formalin-fixed and paraffin-embedded human tonsil tissue reacted with BPI Antibody (Center) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.| Application Notes | ELISA: Use at 1:1,000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at at 2-8°C for up to 6 months. For long term storage, aliquot and store at -20 °C. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.09% (W/V) sodium azide |
