Application
WB analysis of extracts from various cell lines using B-Raf antibody.| Product Name | B-Raf antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody to B-RafThis gene encodes a protein belonging to the raf/mil family of serine/threonine protein kinases. This protein plays a role in regulating the MAP kinase/ERKs signaling pathway, which affects cell division, differentiation, and secretion. Mutations in this gene are associated with cardiofaciocutaneous syndrome, a disease characterized by heart defects, mental retardation and a distinctive facial appearance. Mutations in this gene have also been associated with various cancers, including non-Hodgkin lymphoma, colorectal cancer, malignant melanoma, thyroid carcinoma, non-small cell lung carcinoma, and adenocarcinoma of lung. A pseudogene, which is located on chromosome X, has been identified for this gene. [provided by RefSeq, Jul 2008] |
| Immunogen | Recombinant protein of human BRAF |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Mouse |
| Tested Applications | ICC/IFIHC-PWB |
| WB: 1:500 - 1:2000. IHC: 1:200 - 1:500. ICC: 1:200 - 1:500.: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | NS7 v-raf murine sarcoma viral oncogene homolog B1 RAFB1 BRAF1 BRAF B-RAF1 |
|---|---|
| Target | B-Raf |
| Molecular Weight(MW) | 84 kDa (note) |
Application
WB analysis of extracts from various cell lines using B-Raf antibody.| Application Notes | WB: 1:500 - 1:2000. IHC: 1:200 - 1:500. ICC: 1:200 - 1:500.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C (regular) or -80°C (long term). Avoid freeze / thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
