Application
Anti-BRAF mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected with BRAFApplication
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-BRAF monoclonal antibody.Application
HEK293T cells transfected with either pCMV6-ENTRY BRAFApplication
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BRAF (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BRAF.Application
Immunohistochemical staining of paraffin-embedded Carcinoma of liver tissue using anti-BRAFmouse monoclonal antibody. ( Dilution 1:50)Application
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of endometrium tissue using anti-BRAFmouse monoclonal antibody. ( Dilution 1:50)Application
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of breast tissue using anti-BRAF mouse monoclonal antibody. ( Dilution 1:50)Application
Immunohistochemical staining of paraffin-embedded pancreas tissue using anti-BRAFmouse monoclonal antibody. ( Dilution 1:50)Application
Immunohistochemical staining of paraffin-embedded liver tissue using anti-BRAFmouse monoclonal antibody. ( Dilution 1:50)Application
Immunohistochemical staining of paraffin-embedded Kidney tissue using anti-BRAFmouse monoclonal antibody. ( Dilution 1:50)Application
Immunofluorescent staining of HepG2 cells using anti-BRAF mouse monoclonal antibody .Application
Immunohistochemical staining of paraffin-embedded endometrium tissue using anti-BRAFmouse monoclonal antibody. ( Dilution 1:50)| Product Name | B-Raf antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [1E2] to B-RafInvolved in the transduction of mitogenic signals from the cell membrane to the nucleus. May play a role in the postsynaptic responses of hippocampal neuron. |
| Immunogen | Full length human recombinant protein of human BRAF (NP_004324) produced in HEK293T cell. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 1E2 |
| Host Species | Mouse |
| Tested Applications | FACSICC/IFIHCIHC-PWB |
| IHC 1:50 : |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | B-raf 1 BRAF BRAF1 MGC126806 MGC138284 RAFB1 Braf 1 B raf 1 |
|---|---|
| Target | B-Raf |
| Tissue Specificity | is specific for human BRAF (NP_004324). |
Application
Anti-BRAF mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected with BRAF
Application
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-BRAF monoclonal antibody.
Application
HEK293T cells transfected with either pCMV6-ENTRY BRAF
Application
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BRAF (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BRAF.
Application
Immunohistochemical staining of paraffin-embedded Carcinoma of liver tissue using anti-BRAFmouse monoclonal antibody. ( Dilution 1:50)
Application
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of endometrium tissue using anti-BRAFmouse monoclonal antibody. ( Dilution 1:50)
Application
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of breast tissue using anti-BRAF mouse monoclonal antibody. ( Dilution 1:50)
Application
Immunohistochemical staining of paraffin-embedded pancreas tissue using anti-BRAFmouse monoclonal antibody. ( Dilution 1:50)
Application
Immunohistochemical staining of paraffin-embedded liver tissue using anti-BRAFmouse monoclonal antibody. ( Dilution 1:50)
Application
Immunohistochemical staining of paraffin-embedded Kidney tissue using anti-BRAFmouse monoclonal antibody. ( Dilution 1:50)
Application
Immunofluorescent staining of HepG2 cells using anti-BRAF mouse monoclonal antibody .
Application
Immunohistochemical staining of paraffin-embedded endometrium tissue using anti-BRAFmouse monoclonal antibody. ( Dilution 1:50)| Application Notes | IHC 1:50 : |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4°C or aliquot into working amounts and store at -20°C. For long-term storage, store at -70°C (aliquotted). Avoid repeated freeze-thaw cycles. |
| Storage Buffer | PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide. |
