Application
Western blot analysis using BRAF mouse mAb against HEK293T cells transfected with the pCMV6-ENTRY control (1) and pCMV6-ENTRY Braf cDNA (2).Application
Immunohistochemical analysis of paraffin-embedded human testis tissues using anti-BRAF mAb.Application
Western blot analysis using anti-Braf monoclonal antibody against truncated recombinant Braf (1) and A431 cell lysate (2).Application
Immunohistochemical analysis of paraffin-embedded human bladder carcinoma tissue(left) and lung carcinoma tissue(right) showing cytoplasmic localization using anti-BRAF with DAB staining.| Product Name | B-Raf antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [1H12F1, 1H12G10,1F12F11C9] to B-RafThis gene encodes a protein belonging to the raf/mil family of serine/threonine protein kinases. This protein plays a role in regulating the MAP kinase/ERKs signaling pathway, which affects cell division, differentiation, and secretion. Mutations in this gene are associated with cardiofaciocutaneous syndrome, a disease characterized by heart defects, mental retardation and a distinctive facial appearance. Mutations in this gene have also been associated with various cancers, including non-Hodgkin lymphoma, colorectal cancer, malignant melanoma, thyroid carcinoma, non-small cell lung carcinoma, and adenocarcinoma of lung. A pseudogene, which is located on chromosome X, has been identified for this gene. [provided by RefSeq, Jul 2008] |
| Immunogen | The immunogen corresponding to BRAF expressed in E. Coli. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 1H12F1, 1H12G10,1F12F11C9 |
| Host Species | Mouse |
| Tested Applications | ELISAIHCIHC-PWB |
| ELISA: 1/10000.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | B-RAF1 BRAF1 BRAF NS7 RAFB1 |
|---|---|
| Target | B-Raf |
Application
Western blot analysis using BRAF mouse mAb against HEK293T cells transfected with the pCMV6-ENTRY control (1) and pCMV6-ENTRY Braf cDNA (2).
Application
Immunohistochemical analysis of paraffin-embedded human testis tissues using anti-BRAF mAb.
Application
Western blot analysis using anti-Braf monoclonal antibody against truncated recombinant Braf (1) and A431 cell lysate (2).
Application
Immunohistochemical analysis of paraffin-embedded human bladder carcinoma tissue(left) and lung carcinoma tissue(right) showing cytoplasmic localization using anti-BRAF with DAB staining.| Application Notes | ELISA: 1/10000.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4℃, for long term storage, store at -20℃. |
| Storage Buffer | Ascitic fluid containing 0.03% sodium azide. |
