Application
HEK293T cells transfected with either BRAF overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-BRAF antibody , and then analyzed by flow cytometry.Application
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BRAF (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BRAF.Application
Immunohistochemical staining of paraffin-embedded Human pancreas tissue using anti-BRAF mouse monoclonal antibody.Application
Immunohistochemical staining of paraffin-embedded Human lung tissue using anti-BRAF mouse monoclonal antibody.Application
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-BRAF monoclonal antibody.Application
Immunohistochemical staining of paraffin-embedded Human endometrium tissue using anti-BRAF mouse monoclonal antibody.Application
Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-BRAF mouse monoclonal antibody.Application
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-BRAF mouse monoclonal antibody.Application
Anti-BRAF mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected with BRAF| Product Name | B-Raf antibody [5A9] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [5A9] to B-RafInvolved in the transduction of mitogenic signals from the cell membrane to the nucleus. May play a role in the postsynaptic responses of hippocampal neuron. |
| Immunogen | Protein expressed in 293T cell transfected with human BRAF expression vector |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 5A9 |
| Host Species | Mouse |
| Tested Applications | FACS,ICC/IF,IHC,IHC-P,WB |
| IHC 1:50 | |
| Species Reactivity | Human,Monkey |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | B-raf 1, BRAF, BRAF1, MGC126806, MGC138284, RAFB1, Braf 1, B raf 1 |
|---|---|
| Target | B-Raf |
| Tissue Specificity | is specific for human BRAF. |
Application
HEK293T cells transfected with either BRAF overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-BRAF antibody , and then analyzed by flow cytometry.
Application
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BRAF (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BRAF.
Application
Immunohistochemical staining of paraffin-embedded Human pancreas tissue using anti-BRAF mouse monoclonal antibody.
Application
Immunohistochemical staining of paraffin-embedded Human lung tissue using anti-BRAF mouse monoclonal antibody.
Application
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-BRAF monoclonal antibody.
Application
Immunohistochemical staining of paraffin-embedded Human endometrium tissue using anti-BRAF mouse monoclonal antibody.
Application
Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-BRAF mouse monoclonal antibody.
Application
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-BRAF mouse monoclonal antibody.
Application
Anti-BRAF mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected with BRAF| Application Notes | IHC 1:50 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4°C or aliquot into working amounts and store at -20°C. For long-term storage, store at -70°C (aliquotted). Avoid repeated freeze-thaw cycles. |
| Storage Buffer | PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide. |
