Application
Sample(30 ug whole cell lysate) A: 293T 12% SDS PAGE diluted at 1:500Application
BRAF35 antibody detects BRAF35 protein at nucleus by immunofluorescent analysis. Sample: A375 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: BRAF35 protein stained by BRAF35 antibody diluted at 1:500. Blue: Hoechst 33342 staining.Application
BRAF35 antibody immunoprecipitates BRAF35 protein in IP experiments. IP Sample: 293T whole cell lysate/extract A. 40 μg 293T whole cell lysate/extract B. Control with 2 μg of preimmune rabbit IgG C. Immunoprecipitation of BRAF35 protein by 2 μg of BRAF35 antibody 10% SDS-PAGE The immunoprecipitated BRAF35 protein was detected by BRAF35 antibody diluted at 1:1000. EasyBlot anti-rabbit IgG was used as a secondary reagent.| Product Name | BRAF35 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to BRAF35 (high-mobility group 20B)Required for correct progression through G2 phase of the cell cycle and entry into mitosis. Required for RCOR1/CoREST mediated repression of neuronal specific gene promoters. |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human BRAF35. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IF,IP,WB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, BovineMouse (95%), Rat (95%), Bovine (98%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | BRAF25 antibody, BRAF35 antibody, FLJ26127 antibody, HMGX2 antibody, HMGXB2 antibody, PP7706 antibody, SMARCE1r antibody, SOXL antibody, pp8857 antibody, HMG20B antibody, high-mobility group 20B antibody, HMG box-containing protein 20B antibody, SWI/SNF-re |
|---|---|
| Molecular Weight(MW) | 36 kDa (note) |
| Cellular Localization | Nucleus |
Application
Sample(30 ug whole cell lysate) A: 293T 12% SDS PAGE diluted at 1:500
Application
BRAF35 antibody detects BRAF35 protein at nucleus by immunofluorescent analysis. Sample: A375 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: BRAF35 protein stained by BRAF35 antibody diluted at 1:500. Blue: Hoechst 33342 staining.
Application
BRAF35 antibody immunoprecipitates BRAF35 protein in IP experiments. IP Sample: 293T whole cell lysate/extract A. 40 μg 293T whole cell lysate/extract B. Control with 2 μg of preimmune rabbit IgG C. Immunoprecipitation of BRAF35 protein by 2 μg of BRAF35 antibody 10% SDS-PAGE The immunoprecipitated BRAF35 protein was detected by BRAF35 antibody diluted at 1:1000. EasyBlot anti-rabbit IgG was used as a secondary reagent.| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
