Application
IHC-P analysis of human breast carcinoma tissue using BRCA1 (phospho Ser1423) antibody.Application
WB analysis of extracts from untreted or Anisomycin-treated HT29 cells using BRACA1 (phospho Ser1423) antibody.| Product Name | BRCA1 (phospho Ser1423) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to BRCA1 (phospho Ser1423)This gene encodes a nuclear phosphoprotein that plays a role in maintaining genomic stability, and it also acts as a tumor suppressor. The encoded protein combines with other tumor suppressors, DNA damage sensors, and signal transducers to form a large multi-subunit protein complex known as the BRCA1-associated genome surveillance complex (BASC). This gene product associates with RNA polymerase II, and through the C-terminal domain, also interacts with histone deacetylase complexes. This protein thus plays a role in transcription, DNA repair of double-stranded breaks, and recombination. Mutations in this gene are responsible for approximately 40% of inherited breast cancers and more than 80% of inherited breast and ovarian cancers. Alternative splicing plays a role in modulating the subcellular localization and physiological function of this gene. Many alternatively spliced transcript variants, some of which are disease-associated mutations, have been described for this gene, but the f |
| Immunogen | The antiserum was produced against synthesized phosphopeptide derived from human BRCA1 around the phosphorylation site of serine 1423. |
| Modification | p-Ser1423 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-P,WB |
| WB: 1:500-1:1000 IHC: 1:50-1:100 | |
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | PSCP , RNF53 , PPP1R53 , BRCC1 , PNCA4 , BRCAI , breast cancer 1, early onset , IRIS , BROVCA1 |
|---|---|
| Target | BRCA1 |
| Tissue Specificity | This antibody detects endogenous levels of BRCA1 only when phosphorylated at serine 1423. |
Application
IHC-P analysis of human breast carcinoma tissue using BRCA1 (phospho Ser1423) antibody.
Application
WB analysis of extracts from untreted or Anisomycin-treated HT29 cells using BRACA1 (phospho Ser1423) antibody.| Application Notes | WB: 1:500-1:1000 IHC: 1:50-1:100 |
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| Form | Liquid |
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| Storage Instructions | Aliquot and store at -20°C or -80°C. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
