Application
WB analysis of extracts from untreated and UV-treated HeLa cells using BRCA1 (phospho Ser988) antibody.Application
ICC/IF staining of methanol- fixed HeLa cells using BRCA1 (phospho Ser988) antibody (red) and DAPI (blue).| Product Name | BRCA1 (phospho Ser988) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to BRCA1 (phospho Ser988)This gene encodes a nuclear phosphoprotein that plays a role in maintaining genomic stability, and it also acts as a tumor suppressor. The encoded protein combines with other tumor suppressors, DNA damage sensors, and signal transducers to form a large multi-subunit protein complex known as the BRCA1-associated genome surveillance complex (BASC). This gene product associates with RNA polymerase II, and through the C-terminal domain, also interacts with histone deacetylase complexes. This protein thus plays a role in transcription, DNA repair of double-stranded breaks, and recombination. Mutations in this gene are responsible for approximately 40% of inherited breast cancers and more than 80% of inherited breast and ovarian cancers. Alternative splicing plays a role in modulating the subcellular localization and physiological function of this gene. Many alternatively spliced transcript variants, some of which are disease-associated mutations, have been described for this gene, but the f |
| Immunogen | A phospho specific peptide corresponding to residues surrounding S988 of human BRCA1 |
| Modification | p-Ser988 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IF,WB |
| WB: 1:500 - 1:2000. IF: 1:100 - 1:200. | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | BRCC1 , PSCP , IRIS , RNF53 , breast cancer 1, early onset , BRCAI , PNCA4 , BROVCA1 , BRCA1 , PPP1R53 |
|---|---|
| Target | BRCA1 (phospho Ser988) |
| Molecular Weight(MW) | 220 kDa (note) |
Application
WB analysis of extracts from untreated and UV-treated HeLa cells using BRCA1 (phospho Ser988) antibody.
Application
ICC/IF staining of methanol- fixed HeLa cells using BRCA1 (phospho Ser988) antibody (red) and DAPI (blue).| Application Notes | WB: 1:500 - 1:2000. IF: 1:100 - 1:200. |
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| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C (regular) or -80°C (long term). Avoid freeze / thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
