Application
Western blot analysis of extracts from 293 cells using BRCA2 (Phospho-Ser3291) Antibody. The lane on the right is treated with the antigen-specific peptide.| Product Name | BRCA2 (phospho Ser3291) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to BRCA2 (phospho Ser3291)Inherited mutations in BRCA1 and this gene, BRCA2, confer increased lifetime risk of developing breast or ovarian cancer. Both BRCA1 and BRCA2 are involved in maintenance of genome stability, specifically the homologous recombination pathway for double-strand DNA repair. The BRCA2 protein contains several copies of a 70 aa motif called the BRC motif, and these motifs mediate binding to the RAD51 recombinase which functions in DNA repair. BRCA2 is considered a tumor suppressor gene, as tumors with BRCA2 mutations generally exhibit loss of heterozygosity (LOH) of the wild-type allele. [provided by RefSeq, Dec 2008] |
| Immunogen | Peptide sequence around phosphorylation site of Serine 3291 (F-V-S(p)-P-A) derived from Human BRCA2. |
| Modification | p-Ser3291 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB: 1:500 - 1:1000 | |
| Species Reactivity | Human,Mouse,Rat |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | PNCA2 , BROVCA2 , FANCD1 , breast cancer 2, early onset , XRCC11 , GLM3 , FANCD , FAD1 , FACD , BRCC2 , FAD , BRCA2 |
|---|---|
| Target | BRCA2 (phospho Ser3291) |
| Tissue Specificity | The antibody detects endogenous levels of BRCA2 only when phosphorylated at serine 3291. |
Application
Western blot analysis of extracts from 293 cells using BRCA2 (Phospho-Ser3291) Antibody. The lane on the right is treated with the antigen-specific peptide.| Application Notes | WB: 1:500 - 1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20˚C/1 year |
| Storage Buffer | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
