Rabbit polyclonal antibody to BRCAA1The novel BRCAA1 proteinis epitope, SSKKQKRSHK, was mapped to the cytoplasm of MCF-7 cells. Immunohistochemistry analysis showed that the antigen epitope exhibited positive expression in 65% of breast cancer specimens. Its expression is associated with characteristics that are associated with prognosis of breast cancer, and its sera antibody level may be helpful for breast cancer diagnosis.
Immunogen
Reacts with residues 1046-1055 [SSKKQKRSHK] of the 136 kDa human, mouse and rat BRCAA1 protein.
Key Feature
Clonality
Polyclonal
Host Species
Rabbit
Tested Applications
WB
This antibody may be used for Western blot analysis where a band is seen at ~130 kDa. No other applications have been tested.Suggested Working Dilutions:*Western blot n 1:500 to 1:1,000 *The investigator should determine the optimal dilution for a specific application.
Species Reactivity
Human
Concentration
1mg/ml (Please refer to the vial label for the specific concentration)
Purification
Unpurified
Target Information
Gene Synonyms
RBP1 like protein, Rb binding protein homolog, SIN3A associated protein 180, RBP1like protein, Rbbinding protein homolog, SIN3Aassociated protein 180
Target
BRCAA1
Application
Application
Western Blot: BRCAA1 Antibody [ - Detection of BRCAA1 in MCF-7 whole cell lysate (15 ug) using 1:1,000 dilution of
Application Notes
This antibody may be used for Western blot analysis where a band is seen at ~130 kDa. No other applications have been tested.Suggested Working Dilutions:*Western blot n 1:500 to 1:1,000 *The investigator should determine the optimal dilution for a specific application.
Additional Information
Form
Liquid
Storage Instructions
Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles.
Storage Buffer
0.1 mg in 0.1 ml (1 mg/ml) in PBS containing 0.02% sodium azide.