Application
Immunofluorescence of BRCC36 in Human Brain cells with BRCC36 antibody at 20 ug/mL.Application
Western blot analysis of BRCC36 in MCF7 cell lysate with BRCC36 antibody at (A) 0.5, (B) 1 and (C) 2 ug/mL.Application
Immunohistochemistry of BRCC36 in human brain tissue with BRCC36 antibody at 2.5 ug/mL.| Product Name | BRCC36 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to BRCC36This gene encodes a subunit of the BRCA1-BRCA2-containing complex (BRCC), which is an E3 ubiquitin ligase. This complex plays a role in the DNA damage response, where it is responsible for the stable accumulation of BRCA1 at DNA break sites. The component encoded by this gene can specifically cleave Lys 63-linked polyubiquitin chains, and it regulates the abundance of these polyubiquitin chains in chromatin. The loss of this gene results in abnormal angiogenesis and is associated with syndromic moyamoya, a cerebrovascular angiopathy. Alternative splicing results in multiple transcript variants. A related pseudogene has been identified on chromosome 5. [provided by RefSeq, Jun 2011] |
| Immunogen | BRCC36 antibody was raised against a 18 amino acid synthetic peptide from near the amino terminus of human BRCC36. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IF,IHC-P,WB |
| WB: 0.5 - 2 ug/ml. IHC-P 2.5 ug/ml. IF: 20 ug/ml | |
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | BRCA1/BRCA2-containing complex, subunit 3 , RP11-143H17.2 , C6.1A , BRCC3 , CXorf53 |
|---|---|
| Target | BRCC36 |
Application
Immunofluorescence of BRCC36 in Human Brain cells with BRCC36 antibody at 20 ug/mL.
Application
Western blot analysis of BRCC36 in MCF7 cell lysate with BRCC36 antibody at (A) 0.5, (B) 1 and (C) 2 ug/mL.
Application
Immunohistochemistry of BRCC36 in human brain tissue with BRCC36 antibody at 2.5 ug/mL.| Application Notes | WB: 0.5 - 2 ug/ml. IHC-P 2.5 ug/ml. IF: 20 ug/ml |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Be stored at 4oC for three months and -20oC, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | PBS containing 0.02% sodium azide. |
