Application
BRCC45 antibody detects BRE protein by Western blot analysis. A. 30 ug C8D30 whole cell lysate/extract 10 % SDS-PAGE BRCC45 antibody dilution: 1:1000Application
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using BRE antibody at 1:200 dilution.Application
BRE antibody immunoprecipitates BRE protein in IP experiments. IP Sample: 1000 μg 293T whole cell lysate/extract A. Control with 2.5 μg of preimmune rabbit IgG B. Immunoprecipitation of BRE protein by 2.5 μg of BRE antibody 10% SDS-PAGE The immunoprecipitated BRE protein was detected by BRE antibody diluted at 1:1000. EasyBlot anti-rabbit IgG was used as a secondary reagent.Application
BRCC45 antibody detects BRE protein by Western blot analysis. A. 30 µg 293T whole cell lysate/extract B. 30 µg A431 whole cell lysate/extract 10 % SDS-PAGE BRCC45 antibody dilution: 1:1000| Product Name | BRCC45 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to BRCC45 (brain and reproductive organ-expressed (TNFRSF1A modulator))Component of the BRCA1-A complex, a complex that specifically recognizes 'Lys-63'-linked ubiquitinated histones H2A and H2AX at DNA lesions sites, leading to target the BRCA1-BARD1 heterodimer to sites of DNA damage at double-strand breaks (DSBs). The BRCA1-A complex also possesses deubiquitinase activity that specifically removes 'Lys-63'-linked ubiquitin on histones H2A and H2AX. In the BRCA1-A complex, it acts as an adapter that bridges the interaction between MERIT40/NBA1 and the rest of the complex, thereby being required for the complex integrity and modulating the E3 ubiquitin ligase activity of the BRCA1-BARD1 heterodimer. Probably also plays a role as a component of the BRISC complex, a multiprotein complex that specifically cleaves 'Lys-63'-linked ubiquitin. May play a role in homeostasis or cellular differentiation in cells of neural, epithelial and germline origins. May also act as a death receptor-associated anti-apoptotic protein, which inhibits the mitochondrial apoptotic pathway. May regulate TNF-alpha signaling through its interactions with TNFRSF1A; however these effects may be indirect. |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human BRCC45. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IF,IP,WB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | Human,Mouse |
| Predicted Reactivity | Rat, Zebrafish, Xenopus laevis, Chicken, BovineRat (98%), Zebrafish (85%), Xenopus laevis (92%), Chicken (97%), Bovine (99%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | BRCC4 antibody, BRCC45 antibody, BRE antibody, BRCA1/BRCA2-containing complex subunit 45 antibody, BRCA1/BRCA2-containing complex, subunit 4 antibody, BRCA1-A complex subunit BRE antibody, brain and reproductive organ-expressed protein antibody, brain and |
|---|---|
| Molecular Weight(MW) | 44 kDa (note) (10) |
| Cellular Localization | Cytoplasm , Nucleus |
Application
BRCC45 antibody detects BRE protein by Western blot analysis. A. 30 ug C8D30 whole cell lysate/extract 10 % SDS-PAGE BRCC45 antibody dilution: 1:1000
Application
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using BRE antibody at 1:200 dilution.
Application
BRE antibody immunoprecipitates BRE protein in IP experiments. IP Sample: 1000 μg 293T whole cell lysate/extract A. Control with 2.5 μg of preimmune rabbit IgG B. Immunoprecipitation of BRE protein by 2.5 μg of BRE antibody 10% SDS-PAGE The immunoprecipitated BRE protein was detected by BRE antibody diluted at 1:1000. EasyBlot anti-rabbit IgG was used as a secondary reagent.
Application
BRCC45 antibody detects BRE protein by Western blot analysis. A. 30 µg 293T whole cell lysate/extract B. 30 µg A431 whole cell lysate/extract 10 % SDS-PAGE BRCC45 antibody dilution: 1:1000| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
