Application
BRD7 antibody detects BRD7 protein by western blot analysis. HeLa whole cell extracts and nuclear extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with BRD7 antibody diluted at 1:500.Application
BRD7 antibody [C1C3] detects BRD7 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: BRD7 protein stained by BRD7 antibody [C1C3] diluted at 1:500. Red: phalloidin, a cytoskeleton marker, stained by diluted at 1:200. Blue: Hoechst 33342 staining. Scale bar = 10 μm.| Product Name | BRD7 antibody [C1C3] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to BRD7 (bromodomain containing 7)This gene encodes a protein which is a member of the bromodomain-containing protein family. The product of this gene has been identified as a component of one form of the SWI/SNF chromatin remodeling complex, and as a protein which interacts with p53 and is required for p53-dependent oncogene-induced senescence which prevents tumor growth. Pseudogenes have been described on chromosomes 2, 3, 6, 13 and 14. Alternative splicing results in multiple transcript variants. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human BRD7. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IF,WB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, BovineMouse (85%), Rat (84%), Bovine (88%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | BP75 antibody, CELTIX1 antibody, NAG4 antibody, BRD7 antibody, bromodomain-containing protein 7 antibody, protein CELTIX-1 antibody, 75 kDa bromodomain protein antibody, bromodomain containing 7 antibody |
|---|---|
| Molecular Weight(MW) | 74 kDa (note) (2) |
| Cellular Localization | Isoform 2: Nucleus |
Application
BRD7 antibody detects BRD7 protein by western blot analysis. HeLa whole cell extracts and nuclear extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with BRD7 antibody diluted at 1:500.
Application
BRD7 antibody [C1C3] detects BRD7 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: BRD7 protein stained by BRD7 antibody [C1C3] diluted at 1:500. Red: phalloidin, a cytoskeleton marker, stained by diluted at 1:200. Blue: Hoechst 33342 staining. Scale bar = 10 μm.| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
