Application
BrdU antibody detects Bromodeoxyuridine (BrdU) at nucleus in mouse BrdU treated intestine by immunohistochemical analysis. Sample: Paraffin-embedded mouse intestinal tissue pulsed with BrdU. BrdU antibody diluted at 1:500.Application
BrdU antibody detects BrdU protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: BrdU protein stained by BrdU antibody diluted at 1:2000. Blue: Hoechst 33342 staining. Scale bar = 10 μm.Application
BrdU antibody detects BrdU protein from HeLa treated with 10 μM BrdU for 30 minutes and fixed in 4% paraformaldehyde at 4ºC for 15 minutes by flow cytometry analysis. Black: Unlabelled sample was used as a control. Red: BrdU antibody dilution: 1:50. Acquisition of 20,000 events were collected using a Dylight 488-conjugated secondary antibody for FACS analysis.| Product Name | BrdU antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to BrdU |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FACS,ICC/IF,IHC,IHC-F,IHC-P |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
Application
BrdU antibody detects Bromodeoxyuridine (BrdU) at nucleus in mouse BrdU treated intestine by immunohistochemical analysis. Sample: Paraffin-embedded mouse intestinal tissue pulsed with BrdU. BrdU antibody diluted at 1:500.
Application
BrdU antibody detects BrdU protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: BrdU protein stained by BrdU antibody diluted at 1:2000. Blue: Hoechst 33342 staining. Scale bar = 10 μm.
Application
BrdU antibody detects BrdU protein from HeLa treated with 10 μM BrdU for 30 minutes and fixed in 4% paraformaldehyde at 4ºC for 15 minutes by flow cytometry analysis. Black: Unlabelled sample was used as a control. Red: BrdU antibody dilution: 1:50. Acquisition of 20,000 events were collected using a Dylight 488-conjugated secondary antibody for FACS analysis.| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
