Application
Sample (30 ug of whole cell lysate) A: A549 B: Hela C: HCT116 10% SDS PAGE diluted at 1:1000Application
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using BRF2 antibody at 1:200 dilution.Application
Immunohistochemical analysis of paraffin-embedded Ca922 xenograft, using BRF2 antibody at 1:500 dilution.| Product Name | BRF2 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to BRF2 (BRF2, subunit of RNA polymerase III transcription initiation factor, BRF1-like)This gene encodes one of the multiple subunits of the RNA polymerase III transcription factor complex required for transcription of genes with promoter elements upstream of the initiation site. The product of this gene, a TFIIB-like factor, is directly recruited to the TATA-box of polymerase III small nuclear RNA gene promoters through its interaction with the TATA-binding protein. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human BRF2. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IF,IHC-P,WB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, BovineMouse (88%), Rat (90%), Bovine (91%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | BRFU antibody, FLJ11052 antibody, TFIIIB50 antibody, BRF2 antibody, BRF-2 antibody, hBRFU antibody, B-related factor 2 antibody, hTFIIIB50 antibody, transcription factor IIIB 50 kDa subunit antibody, RNA polymerase III transcription initiation factor BRFU |
|---|---|
| Molecular Weight(MW) | 47 kDa (note) (2) |
Application
Sample (30 ug of whole cell lysate) A: A549 B: Hela C: HCT116 10% SDS PAGE diluted at 1:1000
Application
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using BRF2 antibody at 1:200 dilution.
Application
Immunohistochemical analysis of paraffin-embedded Ca922 xenograft, using BRF2 antibody at 1:500 dilution.| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
