Application
Western blot analysis of extracts of various cell lines, using SMARCA4 antibody.Application
Immunofluorescence analysis of A549 cell using SMARCA4 antibody. Blue: DAPI for nuclear staining.Application
Immunofluorescence analysis of HeLa cell using SMARCA4 antibody.Application
Immunohistochemistry of paraffin-embedded human colon carcinoma using SMARCA4 antibody at dilution of 1:100 (x40 lens).Application
Immunohistochemistry of paraffin-embedded human colon using SMARCA4 antibody at dilution of 1:100 (x40 lens).| Product Name | BRG1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to BRG1The protein encoded by this gene is a member of the SWI/SNF family of proteins and is similar to the brahma protein of Drosophila. Members of this family have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The encoded protein is part of the large ATP-dependent chromatin remodeling complex SNF/SWI, which is required for transcriptional activation of genes normally repressed by chromatin. In addition, this protein can bind BRCA1, as well as regulate the expression of the tumorigenic protein CD44. Mutations in this gene cause rhabdoid tumor predisposition syndrome type 2. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2012] |
| Immunogen | A synthetic peptide of human SMARCA4 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IF,IHC,IHC-P,IP,WB |
| WB 1:500 - 1:2000, IHC 1:50 - 1:200, IF 1:50 - 1:200, IP 1:20 - 1:50 | |
| Species Reactivity | Human,Mouse,Rat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | SNF2L4 , SNF2 , BAF190 , SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4 , hSNF2b , RTPS2 , SWI2 , SNF2LB , BAF190A , SMARCA |
|---|---|
| Target | BRG1 |
| Molecular Weight(MW) | 185 kDa (note) |
Application
Western blot analysis of extracts of various cell lines, using SMARCA4 antibody.
Application
Immunofluorescence analysis of A549 cell using SMARCA4 antibody. Blue: DAPI for nuclear staining.
Application
Immunofluorescence analysis of HeLa cell using SMARCA4 antibody.
Application
Immunohistochemistry of paraffin-embedded human colon carcinoma using SMARCA4 antibody at dilution of 1:100 (x40 lens).
Application
Immunohistochemistry of paraffin-embedded human colon using SMARCA4 antibody at dilution of 1:100 (x40 lens).| Application Notes | WB 1:500 - 1:2000, IHC 1:50 - 1:200, IF 1:50 - 1:200, IP 1:20 - 1:50 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
