Application
Immunohistochemical analysis of paraffin-embedded TOV-21G xenograft, using Brn2 antibody at 1:500 dilution.Application
Whole cell extract (30 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with Brn2 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Brn2 antibody immunoprecipitates Brn2 protein-DNA in ChIP experiments. ChIP Sample: 293T whole cell lysate/extract A. 5 μg preimmune rabbit IgG B. 5 μg of Brn2 antibody The precipitated DNA was detected by PCR with primer set targeting to MITF promoter.Application
Brn2 antibody immunoprecipitates Brn2 protein in IP experiments. IP Sample: 1000 µg 293T whole cell lysate/extract A. 50 μg 293T whole cell lysate/extract B. Control with 2 μg of preimmune rabbit IgG C. Immunoprecipitation of Brn2 protein by 2 μg of Brn2 antibody 10% SDS-PAGE The immunoprecipitated Brn2 protein was detected by Brn2 antibody diluted at 1:1000. EasyBlot anti-rabbit IgG was used as a secondary reagent.Application
Brn2 antibody detects Brn2 protein expression at nucleus by immunohistochemical analysis. Sample: Frozen sectioned E13.5 Rat brain. Green: Brn2 protein stained by Brn2 antibody diluted at 1:250. Red: beta Tubulin 3/ TUJ1, a mature neuron marker, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] diluted at 1:500. Blue: Fluoroshield with DAPI .Application
Brn2 antibody detects Brn2 protein at nucleus by immunofluorescent analysis. Sample: SK-N-AS cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Brn2 protein stained by Brn2 antibody diluted at 1:1000. Red: beta Tubulin 3/ Tuj1, a cytoskeleton marker, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] diluted at 1:500. Scale bar = 10 μm.| Product Name | Brn2 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Brn2 (POU class 3 homeobox 2)POU3F2 belongs to a large family of transcription factors that bind to the octameric DNA sequence ATGCAAAT. Most of these proteins share a highly homologous region, referred to as the POU domain, that occurs in several mammalian transcription factors, including the octamer-binding proteins Oct1 (POU2F1; MIM 164175) and Oct2 (POU2F2; MIM 164176) and the pituitary protein Pit1 (PIT1; MIM 173110). Class III POU genes are expressed predominantly in the central nervous system (CNS). It is likely that CNS-specific transcription factors such as these play an important role in mammalian neurogenesis by regulating their diverse patterns of gene expression (Schreiber et al., 1993 [PubMed 8441633]; Atanasoski et al., 1995 [PubMed 7601453]).[supplied by OMIM] |
| Immunogen | Recombinant protein encompassing a sequence within the C-terminus region of human Brn2. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IF,IHC,IHC-F,IHC-P,IP,WB,ChIP |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | Human,Mouse,Rat |
| Predicted Reactivity | Xenopus laevis, Chimpanzee, BovineXenopus laevis (97%), Chimpanzee (100%), Bovine (100%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | BRN2 antibody, OTF7 antibody, POUF3 antibody, POU3F2 antibody, brain-2 antibody, brain-specific homeobox/POU domain protein 2 antibody, nervous system-specific octamer-binding transcription factor N-Oct-3 antibody, octamer-binding protein 7 antibody, octam |
|---|---|
| Molecular Weight(MW) | 47 kDa (note) (2) |
| Cellular Localization | Nucleus |
Application
Immunohistochemical analysis of paraffin-embedded TOV-21G xenograft, using Brn2 antibody at 1:500 dilution.
Application
Whole cell extract (30 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with Brn2 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Brn2 antibody immunoprecipitates Brn2 protein-DNA in ChIP experiments. ChIP Sample: 293T whole cell lysate/extract A. 5 μg preimmune rabbit IgG B. 5 μg of Brn2 antibody The precipitated DNA was detected by PCR with primer set targeting to MITF promoter.
Application
Brn2 antibody immunoprecipitates Brn2 protein in IP experiments. IP Sample: 1000 µg 293T whole cell lysate/extract A. 50 μg 293T whole cell lysate/extract B. Control with 2 μg of preimmune rabbit IgG C. Immunoprecipitation of Brn2 protein by 2 μg of Brn2 antibody 10% SDS-PAGE The immunoprecipitated Brn2 protein was detected by Brn2 antibody diluted at 1:1000. EasyBlot anti-rabbit IgG was used as a secondary reagent.
Application
Brn2 antibody detects Brn2 protein expression at nucleus by immunohistochemical analysis. Sample: Frozen sectioned E13.5 Rat brain. Green: Brn2 protein stained by Brn2 antibody diluted at 1:250. Red: beta Tubulin 3/ TUJ1, a mature neuron marker, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] diluted at 1:500. Blue: Fluoroshield with DAPI .
Application
Brn2 antibody detects Brn2 protein at nucleus by immunofluorescent analysis. Sample: SK-N-AS cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Brn2 protein stained by Brn2 antibody diluted at 1:1000. Red: beta Tubulin 3/ Tuj1, a cytoskeleton marker, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] diluted at 1:500. Scale bar = 10 μm.| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
