Application
Immunofluorescence of BRSK1 in Human Brain cells with BRSK1 antibody at 20 ug/mL.Application
Western blot analysis of BRSK1 in human brain tissue lysate with BRSK1 antibody at (A) 0.5, (B) 1 and (C) 2 ug/ml.Application
Immunohistochemistry of BRSK1 in human brain tissue with BRSK1 antibody at 5 ug/ml.| Product Name | BRSK1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to BRSK1BRSK1 was initially identified as a mammalian homolog to the fission yeast S. pombe Cdr2, a mitosis-regulatory kinase and also shows significant homology to the C. elegans neuronal cell polarity regulator SAD1. BRSK1 is unbiquitously expressed, with highest levels of expression in the brain and testes. Similar to its yeast homolog, BRSK1 is thought to be involved in stress-induced cell cycle arrest. Overexpression of this protein leads to the G2/M arrest in HeLa S2 cells and UV-induced G2/M arrest could be partially abrogated by reduced expression of BRSK1 through the use of siRNA, indicating its role in DNA damage checkpoint function. More recently, it has been shown that both BRSK1 and the related protein BRSK2 are required for mammalian neuronal polarization. While BRSK1- and BRSK2-null mice were viable, double-mutant mice died within two hours of birth. Neurons from these mice showed uniformly-sized neurites as opposed to the normal long axon and multiple shorter dendrites. These neurites also displayed both axonal and dendritic markers. At least two isoforms of BRSK1 are known to exist. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISA,ICC/IF,IHC-P,WB |
| Species Reactivity | Human,Mouse,Rat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | BRSK1, SAD1 kinase, BR serinethreonine kinase1, BR serinethreonine kinase 1, SAD-B, BR serine-threonine kinase 1, SADB, SAD B, BR serine threonine kinase 1 |
|---|---|
| Target | BRSK1 |
| Tissue Specificity | This antibody is specific for BRSK1 |
Application
Immunofluorescence of BRSK1 in Human Brain cells with BRSK1 antibody at 20 ug/mL.
Application
Western blot analysis of BRSK1 in human brain tissue lysate with BRSK1 antibody at (A) 0.5, (B) 1 and (C) 2 ug/ml.
Application
Immunohistochemistry of BRSK1 in human brain tissue with BRSK1 antibody at 5 ug/ml.| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Antibody is supplied in PBS containing 0.02% sodium azide |
