Application
Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with BTF antibody diluted at 1:1000.Application
BTF antibody detects BTF protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: BTF protein stained by BTF antibody diluted at 1:500. Red: phalloidin, a cytoskeleton marker, diluted at 1:50.| Product Name | BTF antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to BTF (BCL2-associated transcription factor 1)This gene encodes a transcriptional repressor that interacts with several members of the BCL2 family of proteins. Overexpression of this protein induces apoptosis, which can be suppressed by co-expression of BCL2 proteins. The protein localizes to dot-like structures throughout the nucleus, and redistributes to a zone near the nuclear envelope in cells undergoing apoptosis. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the C-terminus region of human BTF. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IF,WB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Pig, BovineMouse (95%), Rat (95%), Pig (99%), Bovine (99%) |
| Concentration | 0.2mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | BCL2 associated transcription factor 1 , BCLAF1 , bK211L9.1 |
|---|---|
| Target | BTF |
| Molecular Weight(MW) | 106 kDa (note) (4) |
| Cellular Localization | Cytoplasm , Nucleus |
Application
Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with BTF antibody diluted at 1:1000.
Application
BTF antibody detects BTF protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: BTF protein stained by BTF antibody diluted at 1:500. Red: phalloidin, a cytoskeleton marker, diluted at 1:50.| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
