Application
Sample (30 µg of whole cell lysate) A: Raji 7.5% SDS PAGE diluted at 1:5000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Immunofluorescence analysis of paraformaldehyde-fixed A431, using BTK antibody at 1:500 dilution.| Product Name | BTK antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to BTK (tyrosine-protein kinase BTK)The protein encoded by this gene plays a crucial role in B-cell development. Mutations in this gene cause X-linked agammaglobulinemia type 1, which is an immunodeficiency characterized by the failure to produce mature B lymphocytes, and associated with a failure of Ig heavy chain rearrangement. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human BTK. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IF,WB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Pig, Chicken, BovineMouse (97%), Rat (97%), Pig (98%), Chicken (81%), Bovine (98%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | AGMX1 antibody, AT antibody, ATK antibody, BPK antibody, IMD1 antibody, MGC126261 antibody, MGC126262 antibody, PSCTK1 antibody, XLA antibody, BTK antibody, dominant-negative kinase-deficient Brutons tyrosine kinase antibody, agammaglobulinaemia tyrosine k |
|---|---|
| Molecular Weight(MW) | 76 kDa (note) (2) |
| Cellular Localization | Cytoplasm , Membrane; Peripheral membrane protein , Nucleus , Cytoplasm , Membrane , Nucleus |
Application
Sample (30 µg of whole cell lysate) A: Raji 7.5% SDS PAGE diluted at 1:5000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Immunofluorescence analysis of paraformaldehyde-fixed A431, using BTK antibody at 1:500 dilution.| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
