Application
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with BTK antibody diluted at 1:5000.| Product Name | BTK antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to BTK (Bruton agammaglobulinemia tyrosine kinase)The protein encoded by this gene plays a crucial role in B-cell development. Mutations in this gene cause X-linked agammaglobulinemia type 1, which is an immunodeficiency characterized by the failure to produce mature B lymphocytes, and associated with a failure of Ig heavy chain rearrangement. [provided by RefSeq] |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of human BTK. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Pig, BovineMouse (100%), Rat (100%), Pig (100%), Bovine (100%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | Bruton agammaglobulinemia tyrosine kinase , ATK , PSCTK1 , BPK , AGMX1 , IMD1 , XLA , AT |
|---|---|
| Target | BTK |
| Molecular Weight(MW) | 76 kDa (note) (3) |
| Cellular Localization | Cytoplasm , Membrane; Peripheral membrane protein , Nucleus , Cytoplasm , Membrane , Nucleus |
Application
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with BTK antibody diluted at 1:5000.| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
