Application
Western blot analysis of extracts of various cell lines, using BTK antibody.Application
Immunofluorescence analysis of HeLa cell using BTK antibody. Blue: DAPI for nuclear staining.Application
Immunohistochemistry of paraffin-embedded mouse spleen using BTK antibody at dilution of 1:100 (400x lens).| Product Name | BTK antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to BTKThe protein encoded by this gene plays a crucial role in B-cell development. Mutations in this gene cause X-linked agammaglobulinemia type 1, which is an immunodeficiency characterized by the failure to produce mature B lymphocytes, and associated with a failure of Ig heavy chain rearrangement. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Dec 2013] |
| Immunogen | Recombinant protein of human BTK |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IF,IHC,IHC-P,WB |
| WB 1:500 - 1:2000, IHC 1:50 - 1:200, IF 1:50 - 1:100 | |
| Species Reactivity | Human,Mouse,Rat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | PSCTK1 , XLA , AGMX1 , ATK , IMD1 , Bruton agammaglobulinemia tyrosine kinase , BPK , AT |
|---|---|
| Target | BTK |
| Molecular Weight(MW) | 76 kDa (note) |
Application
Western blot analysis of extracts of various cell lines, using BTK antibody.
Application
Immunofluorescence analysis of HeLa cell using BTK antibody. Blue: DAPI for nuclear staining.
Application
Immunohistochemistry of paraffin-embedded mouse spleen using BTK antibody at dilution of 1:100 (400x lens).| Application Notes | WB 1:500 - 1:2000, IHC 1:50 - 1:200, IF 1:50 - 1:100 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
