Application
Flow cytometric Analysis of Hela cells, using anti-BTN1A1 antibody,(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).Application
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BTN1A1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BTN1A1.Application
Anti-BTN1A1 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected with BTN1A1Application
HEK293T cells transfected with either RC223852 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-BTN1A1 antibody, and then analyzed by flow cytometry.| Product Name | BTN1A1 antibody [3A12] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [3A12] to BTN1A1May function in the secretion of milk-fat droplets. May act as a specific membrane-associated receptor for the association of cytoplasmic droplets with the apical plasma membrane. |
| Immunogen | Full length human recombinant protein of human BTN1A1 (NP_001723) produced in HEK293T cell. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 3A12 |
| Host Species | Mouse |
| Tested Applications | FACS,ICC/IF,WB |
| ICC/IF 1:100 | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | BTN1A1, BT, BTN |
|---|---|
| Target | BTN1A1 |
| Tissue Specificity | is specific for human BTN1A1 (NP_001723). |
Application
Flow cytometric Analysis of Hela cells, using anti-BTN1A1 antibody,(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).
Application
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BTN1A1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BTN1A1.
Application
Anti-BTN1A1 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected with BTN1A1
Application
HEK293T cells transfected with either RC223852 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-BTN1A1 antibody, and then analyzed by flow cytometry.| Application Notes | ICC/IF 1:100 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4°C or aliquot into working amounts and store at -20°C. For long-term storage, store at -70°C (aliquotted). Avoid repeated freeze-thaw cycles. |
| Storage Buffer | PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide. |
