Application
Immunofluorescence of Bub1 in A20 cells with Bub1 antibody at 20 ug/mL.Application
Western blot analysis of bub1 in A-20 lysate with bub1 antibody at (A) 2 and (B) 4 ug/mL.Application
Immunocytochemistry of Bub1 in A20 cells with Bub1 antibody at 10 ug/mL.| Product Name | BUB1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to BUB1This gene encodes a serine/threonine-protein kinase that play a central role in mitosis. The encoded protein functions in part by phosphorylating members of the mitotic checkpoint complex and activating the spindle checkpoint. This protein also plays a role in inhibiting the activation of the anaphase promoting complex/cyclosome. This protein may also function in the DNA damage response. Mutations in this gene have been associated with aneuploidy and several forms of cancer. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013] |
| Immunogen | Bub1 antibody was raised against a 19 amino acid synthetic peptide from near the amino terminus of human Bub1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISA,ICC/IF,WB |
| WB: 2 - 4 ug/ml. ICC: 10 ug/ml. IF: 20 ug/ml | |
| Species Reactivity | Mouse |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | BUB1A , BUB1L , BUB1 mitotic checkpoint serine/threonine kinase , hBUB1 |
|---|---|
| Target | BUB1 |
Application
Immunofluorescence of Bub1 in A20 cells with Bub1 antibody at 20 ug/mL.
Application
Western blot analysis of bub1 in A-20 lysate with bub1 antibody at (A) 2 and (B) 4 ug/mL.
Application
Immunocytochemistry of Bub1 in A20 cells with Bub1 antibody at 10 ug/mL.| Application Notes | WB: 2 - 4 ug/ml. ICC: 10 ug/ml. IF: 20 ug/ml |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Be stored at 4oC for three months and -20oC, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | PBS containing 0.02% sodium azide. |
