Application
Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with BUB1B antibody [N2C1], Internal diluted at 1:500.Application
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with BUB1B antibody [N2C1], Internal diluted at 1:500.| Product Name | BUB1B antibody , Internal |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to BUB1B (BUB1 budding uninhibited by benzimidazoles 1 homolog beta (yeast))This gene encodes a kinase involved in spindle checkpoint function. The protein has been localized to the kinetochore and plays a role in the inhibition of the anaphase-promoting complex/cyclosome (APC/C), delaying the onset of anaphase and ensuring proper chromosome segregation. Impaired spindle checkpoint function has been found in many forms of cancer. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human BUB1B. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | Human |
| Predicted Reactivity | BovineBovine (87%) |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | BUB1beta antibody, BUBR1 antibody, Bub1A antibody, MAD3L antibody, SSK1 antibody, hBUBR1 antibody, BUB1B antibody, mitotic checkpoint serine/threonine-protein kinase BUB1 beta antibody, mitotic checkpoint kinase MAD3L antibody, MAD3/BUB1-related protein ki |
|---|---|
| Target | BUB1 beta |
| Molecular Weight(MW) | 120 kDa (note) |
| Cellular Localization | Cytoplasm , Nucleus , Kinetochore |
Application
Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with BUB1B antibody [N2C1], Internal diluted at 1:500.
Application
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with BUB1B antibody [N2C1], Internal diluted at 1:500.| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
