Rabbit Polyclonal antibody to BUB3 (phospho Tyr207)This gene encodes a protein involved in spindle checkpoint function. The encoded protein contains four WD repeat domains and has sequence similarity with the yeast BUB3 protein. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]
Immunogen
Peptide sequence around phosphorylation site of Tyrosine 207 (V-E-Y(p)-L-D) derived from Human Bub3.
Modification
p-Tyr207
Key Feature
Clonality
Polyclonal
Isotype
IgG
Host Species
Rabbit
Tested Applications
WB
WB: 1:1000
Species Reactivity
Human,Mouse,Rat
Concentration
1mg/ml (Please refer to the vial label for the specific concentration)
Purification
Affinity purified
Target Information
Gene Synonyms
BUB3 , hBUB3 , BUB3L , BUB3 mitotic checkpoint protein
Target
BUB3 (phospho Tyr207)
Tissue Specificity
The antibody detects endogenous level of Bub3 only when phosphorylated at Tyrosine 207.
Application
Application
Western blot analysis of Bub3 (Phospho-Tyr207)Antibody (arrow indicated) in U87 (A) and U87 EGFRvIII (B) cells in Mitosis.
Application Notes
WB: 1:1000
Additional Information
Form
Liquid
Storage Instructions
Store at -20°C/1 year
Storage Buffer
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.