Application
Sample (30 ug of whole cell lysate) A: 293T B: H1299 10% SDS PAGE diluted at 1:1000Application
Immunoprecipitation of BUB3 protein from 293T whole cell extracts using 5 μg of BUB3 antibody [N1C1] . Western blot analysis was performed using BUB3 antibody [N1C1] . EasyBlot anti-Rabbit IgG was used as a secondary reagent.Application
Immunohistochemical analysis of paraffin-embedded A549 Xenograft, using BUB3 antibody at 1:100 dilution.Application
BUB3 antibody detects BUB3 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with BUB3 antibody diluted by 1:1000.| Product Name | BUB3 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to BUB3 (budding uninhibited by benzimidazoles 3 homolog (yeast))This gene encodes a protein involved in spindle checkpoint function. The encoded protein contains four WD repeat domains and has sequence similarity with the yeast BUB3 protein. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human BUB3. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-P,IP,WB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Zebrafish, Xenopus laevis, Chicken, Rhesus Monkey, BovineMouse (100%), Rat (100%), Zebrafish (83%), Xenopus laevis (92%), Chicken (96%), Rhesus Monkey (100%), Bovine (100%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | BUB3L antibody, hBUB3 antibody, BUB3 antibody, BUB3 budding uninhibited by benzimidazoles 3 homolog antibody, mitotic checkpoint component antibody, mitotic checkpoint protein BUB3 antibody, budding uninhibited by benomyl antibody, budding uninhibited by b |
|---|---|
| Target | BUB3 |
| Molecular Weight(MW) | 37 kDa (note) (3) |
| Cellular Localization | Nucleus |
Application
Sample (30 ug of whole cell lysate) A: 293T B: H1299 10% SDS PAGE diluted at 1:1000
Application
Immunoprecipitation of BUB3 protein from 293T whole cell extracts using 5 μg of BUB3 antibody [N1C1] . Western blot analysis was performed using BUB3 antibody [N1C1] . EasyBlot anti-Rabbit IgG was used as a secondary reagent.
Application
Immunohistochemical analysis of paraffin-embedded A549 Xenograft, using BUB3 antibody at 1:100 dilution.
Application
BUB3 antibody detects BUB3 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with BUB3 antibody diluted by 1:1000.| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
