Application
WB analysis of (1) NIH-3T3, (2) HepG2, (3) Jurkat, (4) HeLa and (5) SH-SY5Y lysates (40ug per lane) using CRP antibody at a dilution of 1:1,000.| Product Name | C Reactive Protein antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [5A9] to C Reactive ProteinThe protein encoded by this gene belongs to the pentaxin family. It is involved in several host defense related functions based on its ability to recognize foreign pathogens and damaged cells of the host and to initiate their elimination by interacting with humoral and cellular effector systems in the blood. Consequently, the level of this protein in plasma increases greatly during acute phase response to tissue injury, infection, or other inflammatory stimuli. [provided by RefSeq, Sep 2009] |
| Immunogen | Recombinant human CRP (19-224aa) purified from E. coli |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2a |
| Clone Number | 5A9 |
| Host Species | Mouse |
| Tested Applications | ELISAWB |
| The antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis is 1:1,000 ~ 2,000. Recommended starting dilution is 1:1,000.: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | PTX1 C-reactive protein pentraxin-related |
|---|---|
| Target | C Reactive Protein |
| Light Chain Type | kappa |
Application
WB analysis of (1) NIH-3T3, (2) HepG2, (3) Jurkat, (4) HeLa and (5) SH-SY5Y lysates (40ug per lane) using CRP antibody at a dilution of 1:1,000.| Application Notes | The antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis is 1:1,000 ~ 2,000. Recommended starting dilution is 1:1,000.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Can be stored at +4C. For long term storage, aliquot and store at -20C. Avoid repeated freezing and thawing cycles. |
| Storage Buffer | Phosphate-Buffered Saline (pH 7.4) with 0.1% Sodium Azide. |
