Application
Western blot analysis of extracts from HepG2 cells treated with EGF using C/EBP-α (phospho-Ser21) Antibody.The lane on the right is treated with the antigen-specific peptide.Application
Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using C/EBP-α (phospho-Ser21) antibody (left)or the same antibody preincubated with blocking peptide (right).| Product Name | C/EBP alpha (phospho Ser21) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to C/EBP alpha (phospho Ser21)This intronless gene encodes a transcription factor that contains a basic leucine zipper (bZIP) domain and recognizes the CCAAT motif in the promoters of target genes. The encoded protein functions in homodimers and also heterodimers with CCAAT/enhancer-binding proteins beta and gamma. Activity of this protein can modulate the expression of genes involved in cell cycle regulation as well as in body weight homeostasis. Mutation of this gene is associated with acute myeloid leukemia. The use of alternative in-frame non-AUG (GUG) and AUG start codons results in protein isoforms with different lengths. Differential translation initiation is mediated by an out-of-frame, upstream open reading frame which is located between the GUG and the first AUG start codons. [provided by RefSeq, Dec 2013] |
| Immunogen | Peptide sequence around phosphorylation site of Serine 21 (L-Q-S(p)-P-P) derived from Human C/EBP-α. |
| Modification | p-Ser21 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-P,WB |
| WB: 1:500 - 1:1000. IHC: 1:50 - 1:100 | |
| Species Reactivity | Human,Mouse,Rat |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | CCAAT/enhancer binding protein (C/EBP), alpha , CEBPA , CEBP , C/EBP-alpha |
|---|---|
| Target | C/EBP alpha (phospho Ser21) |
| Tissue Specificity | The antibody detects endogenous levels of C/EBP-α only when phosphorylated at serine 21. |
Application
Western blot analysis of extracts from HepG2 cells treated with EGF using C/EBP-α (phospho-Ser21) Antibody.The lane on the right is treated with the antigen-specific peptide.
Application
Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using C/EBP-α (phospho-Ser21) antibody (left)or the same antibody preincubated with blocking peptide (right).| Application Notes | WB: 1:500 - 1:1000. IHC: 1:50 - 1:100 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20˚C/1 year |
| Storage Buffer | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
