Application
Western blot analysis of C/EBP alpha (pT226) expression in MCF7 EGF-treated (A); mouse spleen (B); mouse liver (C); rat spleen (D); rat liver (E) whole cell lysates.| Product Name | C/EBP alpha (phospho Thr226) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to C/EBP alpha (phospho Thr226)This intronless gene encodes a transcription factor that contains a basic leucine zipper (bZIP) domain and recognizes the CCAAT motif in the promoters of target genes. The encoded protein functions in homodimers and also heterodimers with CCAAT/enhancer-binding proteins beta and gamma. Activity of this protein can modulate the expression of genes involved in cell cycle regulation as well as in body weight homeostasis. Mutation of this gene is associated with acute myeloid leukemia. The use of alternative in-frame non-AUG (GUG) and AUG start codons results in protein isoforms with different lengths. Differential translation initiation is mediated by an out-of-frame, upstream open reading frame which is located between the GUG and the first AUG start codons. [provided by RefSeq, Dec 2013] |
| Immunogen | KLH-conjugated synthetic peptide encompassing a sequence within the center region of human C/EBP alpha. The exact sequence is proprietary. |
| Modification | p-Thr226 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB: 1:500 - 1:1000 | |
| Species Reactivity | Human,Mouse,Rat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | CCAAT/enhancer binding protein (C/EBP), alpha , CEBPA , C/EBP-alpha , CEBP |
|---|---|
| Target | C/EBP alpha |
| Tissue Specificity | Recognizes endogenous levels of C/EBP alpha (pT226) protein. |
Application
Western blot analysis of C/EBP alpha (pT226) expression in MCF7 EGF-treated (A); mouse spleen (B); mouse liver (C); rat spleen (D); rat liver (E) whole cell lysates.| Application Notes | WB: 1:500 - 1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4 C. Upon delivery aliquot and store at -20 C for one year. Avoid freeze/thaw cycles. |
| Storage Buffer | Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
