Application
Immunoprecipitation of CEBPB protein from HeLa nuclear extracts using 5 μg of C/EBP beta antibody . Western blot analysis was performed using C/EBP beta antibody . EasyBlot anti-Rabbit IgG was used as a secondary reagent.Application
Cross-linked ChIP was performed with Raji chromatin extract and 5 μg of either control rabbit IgG or anti-C/EBP beta antibody. The precipitated DNA was detected by PCR with primer set targeting to CR2pD.Application
Sample (30 ug of whole cell lysate) A: A431 10% SDS PAGE diluted at 1:1000Application
Immunohistochemical analysis of paraffin-embedded human ovarian cancer, using C/EBP beta antibody at 1:250 dilution.| Product Name | C/EBP beta antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to C/EBP beta (CCAAT/enhancer binding protein (C/EBP), beta)The protein encoded by this intronless gene is a bZIP transcription factor which can bind as a homodimer to certain DNA regulatory regions. It can also form heterodimers with the related proteins CEBP-alpha, CEBP-delta, and CEBP-gamma. The encoded protein is important in the regulation of genes involved in immune and inflammatory responses and has been shown to bind to the IL-1 response element in the IL-6 gene, as well as to regulatory regions of several acute-phase and cytokine genes. In addition, the encoded protein can bind the promoter and upstream element and stimulate the expression of the collagen type I gene. [provided by RefSeq] |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human C/EBP beta. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-P,IP,WB,IHC-R,ChIP |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | Human |
| Predicted Reactivity | PigPig (100%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | C/EBP-beta antibody, CRP2 antibody, IL6DBP antibody, LAP antibody, MGC32080 antibody, NF-IL6 antibody, TCF5 antibody, CEBPB antibody, TCF-5 antibody, CCAAT/enhancer-binding protein beta antibody, liver-enriched transcriptional activator protein antibody, n |
|---|---|
| Molecular Weight(MW) | 36 kDa (note) (2) |
| Cellular Localization | Nucleus |
Application
Immunoprecipitation of CEBPB protein from HeLa nuclear extracts using 5 μg of C/EBP beta antibody . Western blot analysis was performed using C/EBP beta antibody . EasyBlot anti-Rabbit IgG was used as a secondary reagent.
Application
Cross-linked ChIP was performed with Raji chromatin extract and 5 μg of either control rabbit IgG or anti-C/EBP beta antibody. The precipitated DNA was detected by PCR with primer set targeting to CR2pD.
Application
Sample (30 ug of whole cell lysate) A: A431 10% SDS PAGE diluted at 1:1000
Application
Immunohistochemical analysis of paraffin-embedded human ovarian cancer, using C/EBP beta antibody at 1:250 dilution.| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
