C10orf119 antibody detects C10orf119 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with C10orf119 antibody diluted by 1:1000.
Rabbit Polyclonal antibody to C10orf119 (minichromosome maintenance complex binding protein)DNA replication is a carefully orchestrated process involving many proteins that assemble at origins of replication. Among these are the 6 proteins of the minichromosome maintenance (MCM) complex (e.g., MCM2; MIM 116945), which form a hexamer. Each MCM subunit performs an essential function in initiation and elongation of DNA replication. MCMBP can replace MCM2 in the MCM complex, thus forming an alternative MCM hexamer (Sakwe et al., 2007 [PubMed 17296731]).[supplied by OMIM]
Immunogen
Recombinant protein encompassing a sequence within the center region of human C10orf119. The exact sequence is proprietary.
Key Feature
Clonality
Polyclonal
Isotype
IgG
Host Species
Rabbit
Tested Applications
WB
*Optimal dilutions/concentrations should be determined by the researcher.
:
Species Reactivity
Human
Predicted Reactivity
Mouse, Rat, Dog, Pig, Chicken, Rhesus Monkey, BovineMouse (96%), Rat (95%), Dog (97%), Pig (100%), Chicken (90%), Rhesus Monkey (100%), Bovine (99%)
Concentration
1mg/ml (Please refer to the vial label for the specific concentration)
Purification
Affinity purified
Target Information
Gene Synonyms
MCM-BP minichromosome maintenance complex binding protein MCMBP
Target
C10orf119
Molecular Weight(MW)
73 kDa (note) (4)
Application
Application
C10orf119 antibody detects C10orf119 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with C10orf119 antibody diluted by 1:1000.
Positive Control
293T , A431
Application Notes
*Optimal dilutions/concentrations should be determined by the researcher.
:
Additional Information
Form
Liquid
Storage Instructions
Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Storage Buffer
0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.