Application
Western blot analysis of C13orf3 Antibody (Center) in Hela, 293 cell line lysates (35ug/lane). C13orf3 (arrow) was detected using the purified Pab.Application
C13orf3 Antibody (Center) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
C13orf3 Antibody (Center) IHC analysis in formalin fixed and paraffin embedded testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the C13orf3 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.| Product Name | C13orf3 antibody, Internal |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to C13orf3 (Internal)This gene encodes a component of the spindle and kinetochore-associated protein complex that regulates microtubule attachment to the kinetochores during mitosis. The encoded protein localizes to the outer kinetechore and may be required for normal chromosome segregation and cell division. Alternative splicing results in multiple transcript variants. [provided by RefSeq] |
| Immunogen | KLH conjugated synthetic peptide between 232~262 amino acids from the Center region of human C13orf3. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FACS,IHC-P,WB |
| ELISA: Use at 1:1,000 | |
| Species Reactivity | Human |
| Concentration | 0.2mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | Spindle and kinetochore-associated protein 3, SKA3, Spindle and kinetochoreassociated protein3, Spindle and kinetochoreassociated protein 3, SKA3, C13orf3, RAMA1 |
|---|---|
| Target | C13orf3 |
| Molecular Weight(MW) | 46.359 kDa (note) |
| Cellular Localization | Localizes to the outer kinetochore and spindle microtubules during mitosis in a NDC80 complex-dependent manner. |
Application
Western blot analysis of C13orf3 Antibody (Center) in Hela, 293 cell line lysates (35ug/lane). C13orf3 (arrow) was detected using the purified Pab.
Application
C13orf3 Antibody (Center) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
C13orf3 Antibody (Center) IHC analysis in formalin fixed and paraffin embedded testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the C13orf3 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.| Application Notes | ELISA: Use at 1:1,000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at at 2-8°C for up to 6 months. For long term storage, aliquot and store at -20 °C. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.09% (W/V) sodium azide |
