Application
WB analysis of HeLa cells using C14ORF104 antibody at 0.2-1μg/ml.Application
ICC/IF analysis of human nasal epithelial cells using C14ORF104 antibody at 1:500. Cells were fixed with 4% PFA, permeabilized with 0.2% Triton X-100, blocked with 1% skim milk, and incubated with primary antibody at 4 degree C for overnight. A : acetylated alpha-tubulin B : C14ORF104 C : DAPI D : merge| Product Name | C14ORF104 antibody, N-term |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to C14ORF104This gene encodes a highly conserved protein involved in the preassembly of dynein arm complexes which power cilia. These complexes are found in some cilia and are assembled in the cytoplasm prior to transport for cilia formation. Mutations in this gene have been associated with primary ciliary dyskinesia. Multiple transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Oct 2009] |
| Immunogen | A synthetic peptide corresponding to a N-terminal region of Human C14ORF104 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | Human |
| Predicted Reactivity | Cow: 100%; Dog: 93%; Horse: 100%; Human: 100%; Mouse: 100%; Pig: 100%; Rat: 100% |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | C14ORF104 PF13 CILD10 DNAAF2 KTU |
|---|---|
| Target | C14ORF104 |
| Molecular Weight(MW) | 91 KDa (note) |
Application
WB analysis of HeLa cells using C14ORF104 antibody at 0.2-1μg/ml.
Application
ICC/IF analysis of human nasal epithelial cells using C14ORF104 antibody at 1:500. Cells were fixed with 4% PFA, permeabilized with 0.2% Triton X-100, blocked with 1% skim milk, and incubated with primary antibody at 4 degree C for overnight. A : acetylated alpha-tubulin B : C14ORF104 C : DAPI D : merge| Positive Control | HeLa |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. For short-term storage, store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
