Application
Sample (30 ug of whole cell lysate) A: U87-MG 12% SDS PAGE diluted at 1:1000Application
C1orf74 antibody [N2C3] detects C1orf74 protein at cytoplasm by immunofluorescent analysis. Sample: U87-MG cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: C1orf74 protein stained by C1orf74 antibody [N2C3] diluted at 1:1000. Blue: Hoechst 33342 staining.| Product Name | C1orf74 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to C1orf74 (chromosome 1 open reading frame 74) |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human C1orf74. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | FLJ25078 antibody C1orf74 antibody UPF0739 protein C1orf74 antibody chromosome 1 open reading frame 74 antibody |
|---|---|
| Molecular Weight(MW) | 30 kDa (note) (2) |
Application
Sample (30 ug of whole cell lysate) A: U87-MG 12% SDS PAGE diluted at 1:1000
Application
C1orf74 antibody [N2C3] detects C1orf74 protein at cytoplasm by immunofluorescent analysis. Sample: U87-MG cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: C1orf74 protein stained by C1orf74 antibody [N2C3] diluted at 1:1000. Blue: Hoechst 33342 staining.| Positive Control | U87-MG |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
