Application
C3 antibody [C3], C-term detects C3 protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: C3 protein stained by C3 antibody [C3], C-term diluted at 1:200. Blue: Hoechst 33342 staining.Application
C3 antibody detects C3 protein by western blot analysis. Various whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with C3 antibody diluted at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Immunoprecipitation of C3 protein from HepG2 whole cell extracts using 5 μg of C3 antibody [C3], C-term . Western blot analysis was performed using C3 antibody [C3], C-term . EasyBlot anti-Rabbit IgG was used as a secondary reagent.Application
C3 antibody [C3], C-term detects C3 protein at cytoplasm in mouse brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. C3 antibody [C3], C-term diluted at 1:500.| Product Name | C3 antibody , C-term |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to C3 (complement component 3)Complement component C3 plays a central role in the activation of complement system. Its activation is required for both classical and alternative complement activation pathways. People with C3 deficiency are susceptible to bacterial infection. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the C-terminus region of human C3. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PIPWBImmunosubtraction |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | AHUS5 antibody ARMD9 antibody ASP antibody CPAMD1 antibody C3 antibody complement C3 antibody C3 and PZP-like alpha-2-macroglobulin domain-containing protein 1 antibody complement component C3 antibody acylation-stimulating protein cleavage product |
|---|---|
| Molecular Weight(MW) | 187 kDa (note) |
| Cellular Localization | Secreted |
Application
C3 antibody [C3], C-term detects C3 protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: C3 protein stained by C3 antibody [C3], C-term diluted at 1:200. Blue: Hoechst 33342 staining.
Application
C3 antibody detects C3 protein by western blot analysis. Various whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with C3 antibody diluted at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Immunoprecipitation of C3 protein from HepG2 whole cell extracts using 5 μg of C3 antibody [C3], C-term . Western blot analysis was performed using C3 antibody [C3], C-term . EasyBlot anti-Rabbit IgG was used as a secondary reagent.
Application
C3 antibody [C3], C-term detects C3 protein at cytoplasm in mouse brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. C3 antibody [C3], C-term diluted at 1:500.| Positive Control | Huh7 , HepG2 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
