Application
Sample (30 ug of whole cell lysate) A: HepG2 5% SDS PAGE diluted at 1:500Application
Immunohistochemical analysis of paraffin-embedded human hepatoma, using C4 antibody [N1N2-2], N-term antibody at 1:200 dilution.| Product Name | C4 antibody , N-term |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to C4 (complement component 4A (Rodgers blood group))This gene encodes the acidic form of complement factor 4, part of the classical activation pathway. The protein is expressed as a single chain precursor which is proteolytically cleaved into a trimer of alpha, beta, and gamma chains prior to secretion. The trimer provides a surface for interaction between the antigen-antibody complex and other complement components. The alpha chain may be cleaved to release C4 anaphylatoxin, a mediator of local inflammation. Deficiency of this protein is associated with systemic lupus erythematosus and type I diabetes mellitus. This gene localizes to the major histocompatibility complex (MHC) class III region on chromosome 6. Varying haplotypes of this gene cluster exist, such that individuals may have 1, 2, or 3 copies of this gene. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the N-terminus region of human C4. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | Human |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | C4 antibody C4A2 antibody C4A3 antibody C4A4 antibody C4A6 antibody C4S antibody CO4 antibody CPAMD2 antibody MGC164979 antibody RG antibody C4A antibody C3 and PZP-like alpha-2-macroglobulin domain-containing protein 2 antibody C4A anaphylatox |
|---|---|
| Molecular Weight(MW) | 193 kDa (note) (2) |
| Cellular Localization | Secreted |
Application
Sample (30 ug of whole cell lysate) A: HepG2 5% SDS PAGE diluted at 1:500
Application
Immunohistochemical analysis of paraffin-embedded human hepatoma, using C4 antibody [N1N2-2], N-term antibody at 1:200 dilution.| Positive Control | HepG2 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
